Figure 4. Mode of peptide-membrane interaction evaluated using ITC at 37°C.

Panel A–C shows the heat trace of 20 mM POPC∶POPG (3∶1) LUVs injected into 100 µM peptide (38×1 µL injections), except for OAMPX which was titrated using 8 mM LUVs. The corresponding heat of reaction (Q) and accumulated heat of reaction (Q_acc) are shown in panel D–F and G–I respectively. The arrows in panel D–F highlight the endpoint of the secondary process and the solid lines serve to guide the eye.