Table 3. Survival of Salmonella Typhimurium strains on the eggshell surface after 21 days of incubation: Comparison between different doses (10³ and 10⁵ CFU/mL) of infection.

Salmonella Typhimurium strain	Temperature (°C)	Washing status	Dose of infection (CFU/mL)	Eggshell contamination after incubation (Log CFU/eggshell) Mean ± SE	p-value
S. Typhimurium strain 1	20	Washed	103	3.09±0.82	0.30
			105	4.09±0.27
		Unwashed	103	2.51±1.08	0.15
			105	4.41±0.16
	37	Unwashed	103	3.04±1.24	0.26
			105	4.69±0.18
S. Typhimurium strain 2	20	Washed	103	2.69±1.09	0.17
			105	4.49 ±.013
		Unwashed	103	3.58±0.89	1.00
			105	3.58±0.89
	37	Unwashed	103	ND	NA
			105	ND
S. Typhimurium strain 3	20	Washed	103	2.23±0.92	0.39
			105	3.42±0.92
		Unwashed	103	0.89±0.89	0.51
			105	1.90±1.17
	37	Unwashed	103	ND	NA
			105	ND
S. Typhimurium strain 4	20	Washed	103	0.89±0.89	0.02
			105	4.37±0.21
		Unwashed	103	1.56±0.96	0.23
			105	3.22±0.85
	37	Unwashed	103	ND	0.37
			105	0.69±0.69
S. Typhimurium strain 5	20	Washed	103	1.67±1.04	0.75
			105	2.12±0.88
		Unwashed	103	2.59±1.05	0.11
			105	4.47±0.20
	37	Unwashed	103	ND	NA
			105	ND

NA: Not applicable, ND: Not detected.