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. Author manuscript; available in PMC: 2014 Sep 17.
Published in final edited form as: Biochemistry. 2013 Aug 30;52(37):6568–6574. doi: 10.1021/bi401026d

Fig. 4. SDA of single-Cys F27C (A) or T265C (B).

Fig. 4

RSO membrane vesicles containing the designated single-Cys mutant in the C154V background were incubated with TAM1 in the absence (○) or presence (△) of 1 mM NPG. Vesicles containing the same single-Cys mutants in the C154G background were incubated with TAM1 in the absence of sugar (●). Experiments and data analysis were carried out as described in Fig. 2.