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. Author manuscript; available in PMC: 2014 Mar 12.
Published in final edited form as: Biochemistry. 2009 Jun 9;48(22):4852–4857. doi: 10.1021/bi900014d

Figure 3. A22-mediated inhibition of MreB assembly visualized by fluorescence microscopy.

Figure 3

1 µM MreB (20% Alexa-488 labeled) was polymerized in 10 mM imidazole, pH 7.0, 1 mM MgCl2, 1 mM EGTA, 20 mM KCl and 200 µM ATP at 20° C for 1 hr in the absence (A) or presence (B) of 300 µM A22 and imaged directly by epifluorescence microscopy. Scale bar = 10 µm.