Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Mar 12.

Published in final edited form as: Biochemistry. 2009 Jun 9;48(22):4852–4857. doi: 10.1021/bi900014d

Varying concentrations of MreB were polymerized at 4° C overnight in 10 mM imidazole, pH 7.0, 1 mM MgCl₂, 1 mM EGTA, 20 mM KCl and 200 µM ATP in the absence (A) or presence (B) of 300 µM A22. Samples were equilibrated to 20° C for 1 h and the 400 nm light scattering intensity was measured. Linear fits to the data yielded critical concentrations of 500 nM (A) and 2000 nM (B), respectively.

Varying concentrations of MreB were polymerized at 4° C overnight in 10 mM imidazole, pH 7.0, 1 mM MgCl₂, 1 mM EGTA, 20 mM KCl and 200 µM ATP in the absence (A) or presence (B) of 300 µM A22. Samples were equilibrated to 20° C for 1 h and the 400 nm light scattering intensity was measured. Linear fits to the data yielded critical concentrations of 500 nM (A) and 2000 nM (B), respectively.