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The effect of incubation time of EPI on EE in 110/Chol/DSPE-PEG 2000 LUVs containing 300 mM EDTA diammonium solution, pH 4.3. The process was performed at 60°C and the drug-to-lipid ratio was 1:5 w/w. Errors bar are less than 5%,(n = 3).
