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. 2014 Mar 12;9(3):e91323. doi: 10.1371/journal.pone.0091323

Figure 7. GTPγS-binding assay of the catalytic activities of second-loop and double-loop inserted chimeras.

Figure 7

G-protein activation by the bovine Rh second loop inserted GR chimera (a), the double loop inserted GR chimera (b), and the E132Q-induced double loop inserted chimeras (c). Time-dependent GTPγS-binding to transducin was monitored under light (open circle or square) and dark (filled circle or square) conditions. Solid lines in (a) represent the results of GR/Rh133-152, while dotted lines in (a) represent WT GR. Solid lines in (b) represent the results of GR/Rh133-152 + 228-244, while dotted lines in (b) represent the result of GR/Rh228-244. Solid lines in (c) represent the results of GR/Rh133-152 + 225-252 + E132Q, while dotted lines in (c) represent the results of GR/Rh133-152 + 228-244 + E132Q. (d) Comparison of G-protein activation ability by GR/Rh chimeras. GTPγS-binding to transducin was monitored at 10 min under light (open bar) and dark (filled bar) conditions. Data are presented as the means ± S.D. of more than three independent experiments.