. 2014 Mar 12;9(3):e91323. doi: 10.1371/journal.pone.0091323

Table 1. Light-dependent G-protein activation of the wild-type and GR chimeras.

Sample	Catalytic activity^a (μmol/min/mol pigment)
Third loop inserted chimeras
GR wild-type	10±3
GR wild-type + E132Q	5±3
GR/Rh 223-252	63±5
GR/Rh 223-253	85±8
GR/Rh 225-251	128±10
GR/Rh 225-252	138±8
GR/Rh 226-244	107±5
GR/Rh 227-244	115±10
GR/Rh 228-244	97±10
GR/Rh 229-244	83±5
GR/Rh 226-247	100±3
GR/Rh 227-247	115 ±3
GR/Rh 228-247	82±7
GR/Rh 229-247	93±7
Second loop inserted chimeras
GR/Rh 132-152	10±5
GR/Rh 133-152	9±5
GR/Rh 134-152	5±5
Double loop inserted chimeras
GR/Rh 133-152 + 228-244	480±40
GR/Rh 133-152 + 228-244 + E132Q	990±120
GR/Rh 133-152 + 225-252 + E132Q	1070±130

Light-dependent catalytic activity in the GR was calculated by differences in activities in the light and dark conditions as shown in Figure 3 and Figure 6.