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. 2014 Mar 12;9(3):e91323. doi: 10.1371/journal.pone.0091323

Table 1. Light-dependent G-protein activation of the wild-type and GR chimeras.

Sample Catalytic activitya (μmol/min/mol pigment)
Third loop inserted chimeras
GR wild-type 10±3
GR wild-type + E132Q 5±3
GR/Rh 223-252 63±5
GR/Rh 223-253 85±8
GR/Rh 225-251 128±10
GR/Rh 225-252 138±8
GR/Rh 226-244 107±5
GR/Rh 227-244 115±10
GR/Rh 228-244 97±10
GR/Rh 229-244 83±5
GR/Rh 226-247 100±3
GR/Rh 227-247 115 ±3
GR/Rh 228-247 82±7
GR/Rh 229-247 93±7
Second loop inserted chimeras
GR/Rh 132-152 10±5
GR/Rh 133-152 9±5
GR/Rh 134-152 5±5
Double loop inserted chimeras
GR/Rh 133-152 + 228-244 480±40
GR/Rh 133-152 + 228-244 + E132Q 990±120
GR/Rh 133-152 + 225-252 + E132Q 1070±130
a

Light-dependent catalytic activity in the GR was calculated by differences in activities in the light and dark conditions as shown in Figure 3 and Figure 6.