Figure 2. CD34⁺lin⁻CD10⁺CD24⁻ progenitors display functional features of T-cell progenitors after allo-HSCT.

(A) Limiting dilution assay analysis on OP9-DL1 with CD34⁺Lin⁻CD10⁺CD24⁻ cells sorted from peripheral blood of healthy donors (HD) and at day 100 post allo-HSCT in patients without aGVHD, or with grade I aGVHD (Gr 0-1). NS: Not Significant (Mann-Whitney). (B) Principal Component Analysis (PCA) of CD34⁺Lin⁻CD10⁺CD24⁻ (circles), CD34⁺Lin⁻CD10⁻ cells (squares) and CD34⁻ cells (triangles) from 11 HD and 21 allo-HSCT patients using a panel of 22 genes (see Materiel & Methods). (C) PCA of CD34⁺Lin⁻CD10⁺CD24⁻ from healthy donors (HD, open circles) or patients with (green filled circles) or without severe aGVHD (red filled circles).