| DH5α |
F− Φ80lacZΔM15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK−, mK+) phoA supE44 λ– thi-1 gyrA96 relA1
|
Lab collection |
| BL21(DE3) |
F−
ompT hsdSB(rB−, mB−) gal dcm (DE3) |
NEB |
| 86-028NP |
Nontypeable Haemophilus influenzae strain isolated from the nasopharynx of a child being treated for chronic otitis media |
[14]
|
| ΔtoxAvapA
|
Strain 86-028NP with the toxAvapA locus deleted. |
This work |
|
Plasmid
|
Description
|
Source
|
| pET24b |
Bacterial vector for expressing polyhistidine-tagged proteins |
Novagen |
| pBluescript SK+
|
Bacterial cloning vector |
Lab collection |
| pDD849 |
pBluescript SK+ with the 5′ arm of the toxAvapA plasmid. |
This work |
| pDD851 |
pDD849 with the 3′ arm of the toxAvapA plasmid. |
This work |
| pDD857 |
pDD851 with the spectinomycin resistance cassette. |
This work |
| pDD912 |
pET24b with the toxAvapA locus for protein expression. |
This work |
