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. 2014 Mar 12;9(3):e91555. doi: 10.1371/journal.pone.0091555

Figure 7. Tacrolimus intracellular kinetics.

Figure 7

Intracellular accumulation of tacrolimus (0.05 μM) after 15, 30 sec, 1, 5, 10, 30 or 60 min of efflux (N = 6) in (A) CTL HEK293, CTL HEKpcDNA3.1, HEK1199G and HEK1199A and (B) CTL K562, CTL K562pcDNA3.1, K5621199G and K5621199A. Cells were pre-loaded with tac for 120 min before efflux. The intracellular accumulation of Tac in each cell line was normalized by reporting the absolute amounts of Tac (in ng) on the total amount of proteins in cell extracts, expressed in mg. * compared to CTL pcDNA3.1 *p<0.05 **p<0.01 ***p<0.001, # compared to 1199G/WT #p<0.05 ##p<0.01 ###p<0.001.