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Analysis of DYRK1A sequence using NetPhos 2.0 demonstrates three clusters of Ser/Thr with a high probability of phosphorylation localized at 20–180 aa, 480–540 aa, and 630–670 aa (blue bars). MS/MS identified in Dyrk1A isolated from the murine cytoskeletal fraction three phosphorylation sites: S47, S49, and Y321.
