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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Pediatr Nephrol. 2013 Nov 24;29(4):651–657. doi: 10.1007/s00467-013-2661-y

Figure 3.

Figure 3

The Notch12 and Notch21 alleles. (A) Schematic illustration of Notch1 (black) and Notch2 (red) loci before and after the ICD swap. The swapped fragments between the Notch1 and Notch2 loci range from after the transmembrane domain in Exon 28 to the stop codon in Exon 34. The Notch1 ICD encompasses 5,926bp on chromosome 2, ranging from nucleotide +38,103 to +44,028 (numbering the A in ATG as +1) and encoding amino acid 1,750 to 2,531; for Notch2, the ICD encompasses 8,699bp on chromosome 3, ranging from nucleotide +125,048 to +133,746 and encoding amino acid 1,705 to 2,473. G38066C, G38129A and G125011C denote silent single nucleotide mutations introduced into the targeting constructs for pyrosequencing-based ES cell screening and mRNA comparisons. Amino acids in black denote the S3 cleavage sites.