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. Author manuscript; available in PMC: 2014 Mar 12.
Published in final edited form as: Science. 2013 Mar 28;340(6134):857–861. doi: 10.1126/science.1232245

Fig. 1. Histones extracted from human DIPG containing H3F3A K27M (H3.3) or HIST3H1B K27M (H3.1) mutations exhibit decreased H3K27me3.

Fig. 1

(A) Immunoblots of acid-extracted histones from DIPGs of different indicated H3 genotypes. (B) Immunoblots of histones from PDGF-induced gliomas with and without the H3.3K27M transgene. (C) Immunohistochemistry of H3K27me3 in human or murine gliomas containing wild-type H3.3 or H3.3K27M. (D) Immunohistochemistry quantification of nuclei staining positive for H3K27me3 (P=0.01 for human DIPG, and P = 0.0001 for murine gliomas with the unpaired t test).