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. Author manuscript; available in PMC: 2014 Mar 12.
Published in final edited form as: Science. 2013 Mar 28;340(6134):857–861. doi: 10.1126/science.1232245

Fig. 2. H3K27M transgenes cause a global decrease of H3K27me3.

Fig. 2

(A) Immunoblots of whole-cell extract from lentivirus-transduced 293T cells expressing the indicated H3.3 transgenes. (B) Immunoblots of whole extract from H3.3 K27-to-X transduced 293T cells. The antibody against HA (anti-HA) blot shows the relative H3.3 transgene amounts. (C) Immunoblots of anti-HA–immunoprecipitated heterotypic H3.3 K27M or G34R/V mononucleosomes from the indicated H3.3 transgenic 293T cell lines. (D) Immunoblots of anti-HA–immunoprecipitated oligonucleosomes (>95%of four to five nucleosomes in length, with a median of two to three nucleosomes) from the indicated H3.3 transgenic 293T cell lines (vertical black lines indicate where control lanes were removed for clarity).