Figure 4. Predicted RNA secondary structure of Fragment 4 CD44 and target sites of antisense oligonucleotides.

The predicted secondary structure of Fragment 4 (nts 2861–3055) CD44 was generated using the MFOLD program [53]. As shown, the solid lines indicate regions where DD4 (nts 2921–2943), DD7 (nts 2976–2997) and DD10 (nts 3034–3055) antisense oligonucleotides hybridize. The broken line from nts 2980–3009 indicates the missing region of RNA going from P5 to P4 truncated fragments (Figure 2).