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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Dec 26;34(3):526–532. doi: 10.1161/ATVBAHA.113.302544

Figure 5. Effect of CHOP siRNA on 2-ClHA-induced apoptosis.

Figure 5

THP-1 monocytes transfected with scrambled siRNA or CHOP siRNA were treated with 50 μM of 2-ClHA, then PARP cleavage as well as CHOP expression (18h treatment) was determined by Western blots (A) and caspase 3 activity (6h treatment) was measured as described in “Materials and Methods”. (B). Numbers under the blots represent the ratio of the intensity of the response to that of the maximum response in that blot with normalization to the β-actin loading control, and values are the average of ratios from 3 independent experiments. **P<0.01 between treatments that included the addition of 2-ClHA.