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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Dec 26;34(3):526–532. doi: 10.1161/ATVBAHA.113.302544

Figure 6. ROS is involved in 2-ClHA-induced apoptosis.

Figure 6

THP-1 monocytes were pretreated or not with the antioxidants, NAC (1 mM) or GSH (1 mM) for 1h, and then with 50 μM of 2-ClHA for 3h (A), 6h (B) or 18h (C). Extracellular H2O2 levels (A) and caspase 3 activity (B) were determined as described in “Materials and Methods”. N=3. **P<0.01 vs Blank. ##P<0.01 vs 2-ClHA. PARP cleavage and CHOP expression (C) were determined by Western blotting. Numbers under the blots represent the ratio of the intensity of the response to that of the maximum response in that blot with normalization to the β-actin loading control, and values are the average of ratios from 3 independent experiments.