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NLRP3 and ASC inhibition did not attenuate curcumin-induced caspase-1 activation. Mouse MM cells transfected with siNLRP3 or siASC suppressed NLRP3 (A) and ASC (C) mRNA levels, however did not inhibit caspase-1 activity (B and D) when treated with curcumin (40 µM for 24 h). *p value ≤0.05 when compared to control. †p value ≤ 0.05 when compared to siCon + curcumin.
