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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Cell Signal. 2014 Jan 15;26(5):857–867. doi: 10.1016/j.cellsig.2014.01.006

Fig. 5.

Fig. 5

Importin β-specific inhibitor importazole inhibits the nuclear import of TopBP1. (A) Immunofluorescence microscopy analysis of TopBP1 in nucleus in Xenopus egg extracts with the presence or absence of MMC and importazole at 15 minutes and 60 minutes incubation. The “TopBP1” panel indicates its localization in chromatin/nucleus while the “DNA” panel represents chromatin/nucleus. The scale bar represents 10 μm. (B) Sperm chromatin was added to egg extracts supplemented with MMC and importazole. After 60 minutes incubation, nuclei fraction and total egg extract were isolated and examined via immunoblotting analysis for the indicated proteins.