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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Cell Signal. 2014 Jan 15;26(5):857–867. doi: 10.1016/j.cellsig.2014.01.006

Fig. 6.

Fig. 6

The CTM-dependent shuttling TopBP1 into nuclei and onto chromatin is required for checkpoint activation, not due to its requirement in DNA replication. (A) Experimental design of the experiments in this figure. (B) Nuclei fraction and total extract were examined at 90 minutes via immunoblotting as indicated. (C) Chromatin fraction and total extract were examined at 90 minutes via immunoblotting as indicated. (D) DNA synthesis was examined at different time points (minutes) using the similar procedure as Fig. 4E. (E) Quantification analysis of the DNA synthesis of the samples from (D). (F) MMC was used to trigger DNA damage response in different types of egg extracts. Then chromatin fraction and total extract were analyzed via immunoblotting at 90 minutes for the indicated proteins.