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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Int J Cardiol. 2013 Jul 22;168(4):3659–3664. doi: 10.1016/j.ijcard.2013.05.092

Figure 1. Effect of Dz13 in DOTAP/DOPE on SMC proliferation and c-Jun expression in vitro.

Figure 1

Figure 1

(A) SMCs were grown in 96-well plates, and rendered growth quiescent in serum-free medium for 24h, then treated with Dz13 or Dz13.G>C in DOTAP/DOPE. The medium was changed to 5% FBS medium or serum-free medium. Dz13 in DOTAP/DOPE (0.6µM) reduced SMC proliferation using the Xcelligence system compared with Dz13.G>C. SFM denotes serum-free medium. (B) SMCs were grown in 100 mm petri-dishes, and rendered growth quiescent in serum-free medium for 24h, then treated with Dz13 or Dz13.G>C in DOTAP/DOPE. The medium was then changed to 5% FBS medium for 2 h. Dz13 in DOTAP/DOPE reduced c-Jun protein levels compared with Dz13.G>C. Figure shows typical SMC uptake of FAM-labeled Dz13 under 400× magnification 24 h after transfection with DOTAP/DOPE. * indicates P<0.05, ** indicates P<0.01.