Figure 2. Toll-7 Is Required for RVFV-Induced Autophagy.

(A) WT flies (w¹¹¹⁸) were infected with RVFV, and Atg8 expression was monitored by immunoblot 3 dpi.

(B) Fat body images from uninfected or RVFV-challenged flies expressing GFP-Atg8a (YP1-Gal4 > UAS-GFP-Atg8a). The arrow indicates a cell with GFP-Atg8a puncta in an infected animal. Scale bar represents 10 μM.

(C) Percent of fat body cells containing GFP-Atg8a puncta from uninfected or RVFV-infected flies. Mean ± SD; **p < 0.01, Student's t test.

(D) Survival of RVFV-infected Atg18-silenced flies (Act-Gal4 > UAS-Atg18 IR) or control flies (+ > UAS-Atg18 IR). Mean ± SE; p < 0.001, log-rank test.

(E) Representative RNA blot of RVFV RNA from flies depleted of Atg18 in the fat body (YP1-Gal4 > Atg18 IR) or control flies 6 dpi.

(F) Fold increase in RVFV RNA in Atg18-silenced flies. Mean ± SE; **p < 0.01, Student's t test.

(G) Atg8 immunoblot from uninfected or RVFV-infected Toll-7 mutant or control flies.

(H) Atg8 immunoblot from uninfected or RVFV-challenged Toll-7-overexpressing or control flies. I. Flies (hs > +) were challenged with insulin and either RVFV or PBS, and Akt activity was monitored by immunoblot 3 dpi.

(J) RNA blot for RVFV RNA from flies fed either rapamycin (100 μM) (RAPA) or vehicle control (ethanol, ETOH) 6 dpi.

(K) Fold decrease in viral RNA with rapamycin treatment. Mean ± SE; *p < 0.05, Student's t test.

(L) RNA blot for RVFV RNA 6 dpi from Toll-7 mutant or control flies fed either rapamycin or ethanol. All data are representative of at least three independent experiments. Figure 2, see also Figure S2.