Fig. 3.

Involvement of caspase-3,-9 in NO-induced apoptosis of HDPCs. Activation of caspases were assessed by absorbance for caspase-9 activity was measured at 405 nm by an ELISA reader after incubation with LEHD-pNA substrate (200 µM) for 4 hrs at 37℃ (A). Absorbance for caspase-3 activity was measured in the wells at 405 nm by an ELISA reader after incubation with DEVD-pNA substrate (200 µM) for 24 hrs at 37℃ (B). Cells were incubated for 24 hrs with 4 mM SNP in presence or absence of z-VAD-fmk (50 µM) or z-DEVD-fmk (50 µM) and then MST assay was performed (C). The data are represented as a mean±S.D. from triplicate independent experiments. ^**p<0.01 vs. control; ^##p<0.01 vs. SNP-treated group.