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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Virol Methods. 2014 Jan 10;199:17–24. doi: 10.1016/j.jviromet.2014.01.003

Figure 2. Representative flow plots of ICS results for CD4+ T cells by two methods.

Figure 2

The plots show the gating for a representative sample from an HIV-positive individual stimulated for five hours with DMSO, PTE peptide pools for Gag and Env and PMA/Ionomycin. Plots show the co-expression of IFNγ and TNFα by CD4+ T cell as detected by standard ICS (top row) and viral ICS (bottom row).