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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2013 Aug 15;49(10):746–751. doi: 10.1007/s11626-013-9662-9

Figure 1.

Figure 1

(A) Human amniotic fluid stem cell morphology from the mono-culture and co-culture. Human stem cells were culture in the mono-culture and co-culture systems and morphology was observed after 2 weeks. Scale bar 100 μm. (B) Left panel: RT-PCR was performed with FGF10, uroplakin III and cytokeratin 8 gene primers in stem cells co-cultured with bladder cancer cell line LD605. Right panel: Western blot was performed with the stem cells co-cultured and mono-cultured. FGF10 protein and urothelial markers, UPIII and CK8, were examined on the PAGE analysis. (C) Immunofluorescence of bladder markers uroplakin II, uroplakin III and cytokeratin 8 in the co-culture system. Amniotic fluid stem cells cultured alone (mono-culture) served as a control.