Table 1. Anesthetic Properties (Ketamine, Pentobarbital, Midazolam, Etomidate).

Drug	Dose (mg/kg)	Genotype	n	duration of LORR (min)	time to recovery (min)	Total LORR score	LHWR (min)
Ketamine	150	WT	9	25.0 ± 2.4	36.1 ± 2.4	94.8 ± 5.6	0.0 ± 0.0
		α3KO	7	26.7 ± 4.4	42.0 ± 5.3	110.1 ± 14.4	0.0 ± 0.0
	200	WT	9	40.3 ± 4.4	61.7 ± 3.7	168.3 ± 10.3	0.0 ± 0.0
		α3KO	7	34.1 ± 1.1	61.3 ± 3.0	161.6 ± 7.1	0.0 ± 0.0
	250	WT	9	56.8 ± 5.9	83.0 ± 4.1	231.3 ± 11.7	0.0 ± 0.0
		α3KO	7	62.6 ± 6.7	97.3 ± 5.8	269.6 ± 18.3	0.0 ± 0.0
Pentobarbital	40	WT	9	23.2 ± 3.7	26.3 ± 3.5	75.2 ± 10.6	0.0 ± 0.0
		α3KO	9	34.8 ± 6.0	36.8 ± 5.7	107.6 ± 17.0	0.0 ± 0.0
	50	WT	9	64.7 ± 3.6	65.9 ± 3.5	194.4 ± 10.6	0.0 ± 0.0
		α3KO	9	85.0 ± 9.5	86.0 ± 9.5	255.2 ± 28.5	0.0 ± 0.0
	60	WT	9	96.2 ± 3.6	98.2 ± 3.6	290.3 ± 10.7	0.0 ± 0.0
		α3KO	9	126.8 ± 12.2*	129.1 ± 12.3*	382.1 ± 36.7*	25.0 ± 11.6
Midazolam	75	WT	11	73.5 ± 14.1	101.1 ± 7.5	298.8 ± 23.6	0.0 ± 0.0
		α3KO	11	40.8 ± 9.4	53.8 ± 9.9**	159.4 ± 27.3***	0.0 ± 0.0
Etomidate	30	WT	11	88.3 ± 4.3	91.1 ± 4.5	273.7 ± 10.3	0.0 ± 0.0
		α3KO	11	72.7 ± 5.8*	77.2 ± 5.8	224.9 ± 17.6*	0.0 ± 0.0

Data represent the mean ± SEM. Duration of LORR, time to recovery and the global anesthetic score were significantly increased in α3KO mice compared to wild type mice following 60mg/kg pentobarbital (2-way ANOVA with repeated measures followed by post hoc t-tests with Bonferroni correction). Time to recovery and the global anesthetic score were significantly decreased following 75mg/kg midazolam and the duration of LORR and the global anesthetic score were significantly decreased following 30mg/kg etomidate in α3KO mice compared to wild type mice (unpaired t-test)

^*P<0.05,

^**P<0.01 and

^***P<0.001

Footnote: LORR, loss of righting reflex; LHWR, loss of hindlimb withdrawal reflex; WT, wild type.