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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Circulation. 2005 Aug 30;112(9 0):I229–I234. doi: 10.1161/01.CIRCULATIONAHA.104.524306

Figure 3.

Figure 3

Establishment of the bone-like phenotype in the calcified aortic valves. Left column, control diet; middle column, cholesterol diet; right column, cholesterol diet plus atorvastatin. A, Osteopontin immunostain light microscopy; aortic valve on the left and aorta on the right. Arrow points to aortic valve in each figure. All frames 12.5× magnification. B, Osteopontin Western blot and quantification. C, micro-computed tomography 2-dimensional reconstructed slices of each valve (3.4 mm horizontal field of view). D, Confocal microscopy of the calcein labeling in the mineralizing valve.