Fig. 3.

Effects of 3-day T or DHT exposure in the presence and absence of flutamide (F) on hASC commitment through early-stage preadipocyte differentiation. Confluent hASCs were cultured for 3 days in adipogenesis-inducing medium with vehicle alone (control), T (50 nM), DHT (5 nM) and or F (100 nM). Two sets of experiments are separated by the break in the x-axis. A, PPARγ; B, CEBPα; and C, CEBPβ mRNA expressions were quantified by qRT-PCR. Results represent fold changes treated vs. vehicle control samples. *p < 0.001 vs. vehicle control; ^†p < 0.02, ^††p <0.01, T vs. T+F; ^¶p <0.01, ^¶¶p < 0.003, F vs. T+F; ^‡p <0.05, ^‡‡p < 0.005 DHT vs. DHT+F.