Fig. 5.

Effect of 2-hour androgen preincubation of hASCs with or without flutamide (F) on BMP4-induced mRNA expression of PPARγ, CEBP-α, and CEBP-β. Confluent hASCs were first preincubated for 2 hours in regular DMEM medium with or without BMP4 (5 nM), T (50 nM), DHT (5 nM) and F (100 nM). Cells were then incubated in adipogenesis-inducing medium for another 3 days without BMP4, androgen or F. mRNA expression of PPARγ, CEBP-α, and CEBP-β were determined by qRT-PCR: A and D, PPARγ; B and E, CEBP-α; and C and F, CEBP-β. Results represent fold changes treated vs. vehicle control: *p< 0.05, **p <0.005, ***p<0.001 vs. vehicle control; ^†p< 0.05, ^†† p< 0.001 BMP4 vs. BMP4 + T; ^¶p < 0.05, BMP4 + T vs. BMP4 + T + F; ^‡p <0.05, ^‡‡p < 0.005 BMP4 vs. BMP4 + DHT.