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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: J Endocrinol. 2010 Nov 10;208(2):171–182. doi: 10.1677/JOE-10-0338

Table 1.

Primers used in RT-PCR for amplification of mRNA of specific genes

Gene
Primer sequences
Product size (bp)
GAPDH Forward: 5′-TTC ACC ACC ATG GAG AAG GC-3′
Reverse: 5′-GGC ATG GAC TGT GGT CAT GA-3′
237
ERβ Forward: 5′-TCC CTC TTT GCG TTT GGA CTA-3′
Reverse: 5′-TTC CCG GCA GCA CCA GTA ACC-3′
262
ERα Forward: 5′-AAT TCT GAC AAT CGA CGC CAG-3′
Reverse: 5′-GTG CTT CAA CAT TCT CCC TCC TC-3′
344
bax Forward: 5′-GCA GAG AGG ATG GCT GGG GAG A-3′
Reverse: 5′-TCC AGA CAA GCA GCC GCT CAC G-3′
352
bcl-2 Forward: 5′-GGA TGA CTT CTC TCG TCG CTA C-3′
Reverse: 5′-TGC AGA TGC CGG TTC AG-3′
255