Table 1.

Sequences and sizes of primers used for reverse transcriptase polymerase chain reaction (RT–PCR)

Gene	Primer sequences	Product size (bp)
β-actin	F: 5′-TAC AAC CTC CTT GCA GCT CC-3′	630
	R: 5′-GGA TCT TCA TGA GGT AGT CTG TC-3′
ERβ	F: 5′-TCC CTC TTT GCG TTT GGA CTA-3′	262
	R: 5′-TTC CCG GCA GCA CCA GTA ACC-3′
ERα	F: 5′-AAT TCT GAC AAT CGA CGC CAG-3′	344
	R: 5′-GTG CTT CAA CAT TCT CCC TCC TC-3′
IL-1α	F: 5′-AAGATGTCCAACTTCACCTTCAAGGAGAGCCG-3′	491
	R: 5′-AAGATGTCCAACTTCACCTTCAAGGAGAGCCG-3′
IL-1β	F: 5′-CCAGGATGAGGACCCAAGCA-3′	519
	R: 5′-TCCCGACCATTGCTGTTTCC-3′
TNF-α	F: 5′-TACTGAACTTCGGGGTGATCGGTCC-3′	297
	R: 5′-CAGCCTTGTCCCTTGAAGAGAACC-3′
COX-2	F: 5′-CCT GAG TGG GAT GAC GA-3′	207
	R: 5′-CGG ATG CCA GTG ATA GAG-3′
m-calpain	F: 5′-GGG CAG ACC AAC ATC CAC CTC AGC AAA AAC-3′	404
	R: 5′-GTC TCG ATG CTG AAG CCA TCT GAC TTG AT-3′
Calpastatin	F: 5′-AGT AGT TCT GGA CCC AAT G-3′	230
	R: 5′-CCC CAG TAG ACT TCT CTT TC-3′
Bax	F: 5′-GCA GGG AGG ATG GCT GGG GAG A-3′	352
	R: 5′-TCC AGA CAA GCA GCC GCT CAC G-3′
Bcl-2	F: 5′-GGA TGA CTT CTC TCG TCG CTA C-3′	255
	R: 5′-TGC AGA TGC CGG TTC AG-3′