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. 2014 Feb 4;3(2):e145. doi: 10.1038/mtna.2013.73

Figure 1.

Figure 1

Genome organization and target site location of TT-034, a recombinant AAV8 vector encoding three shRNA targeting the HCV genome. (a) The viral genome of AAV8 has been replaced with 3 expression cassettes separated by Pol III termination sequences and stuffer regions. The contents of the expression cassettes are from 5′ to 3′: modified U6-9 Pol III promoter (box A) driving shRNA22 expression targeted to the HCV NS5B region (viral RNA-dependent RNA polymerase), modified U6-1 Pol III promoter (box B) driving shRNA19 expression, also targeted to the NS5B region and modified U6-8 Pol III promoter (box C) driving shRNA6 targeted to the 5′ UTR of HCV. The expression cassettes are flanked by inverted tandem repeats derived from AAV4 at the 5′ end and AAV2 at the 3′ end of the genome. (b) Schematic representation of the expressed hairpin polarity. The guide strand is defined as the strand targeting the (+) RNA HCV genome.