Figure 2.

Length and sequence diversity of siRNA strands processed out of the three HCV-targeting shRNA expressed from TT-034. Following in vitro transduction of Con1b HCV replicon cells with TT-034, small RNA NGS was performed on RNA extracts to determine the diversity of siRNA guide and passenger strands processed out of TT-034–encoded hairpins. (a–c) The distribution of guide (black circle) and passenger (grey X) strand lengths is indicated for each of the three shRNAs encoded in TT-034. (d–f) The cumulative incidence (y axis, logarithmic scale) of distinct 5′ ends (black bars) and 3′ ends (white bars) processed out of the three shRNAs relative to the TT-034–encoded hairpin sequence (x axis) is also shown as an indicator of sequence diversity.