Figure 6.

Mutational events in herpes simplex virus (HSV)-infected human fibroblasts (HF) treated with trichostatin-A (TSA) or interferon (IFN)-α. (a) Polymerase chain reaction (PCR) amplicons of the HSV region containing the HSV1m5 target site were treated with Surveyor nuclease, and digested products were separated on a 3% agarose gel and quantified using ImageJ. mw: molecular weight size ladder. The percent of sequence modification detected by this assay is indicated. (b) HSV1m5 target sequence analysis. The HSV region containing the HSV1m5 target site was PCR amplified from total genomic DNA obtained at 2 days post-AAV transduction. PCR amplicons were cloned and sequenced from individual bacterial colonies. The frequency and nature of the mutations found are presented. N/A, not applicable, Δ: deletion; wt, wild-type target sequence. Bold indicates the four nucleotides constituting the 3′ overhang generated by the DNA DSB.