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. 2014 Mar 6;94(3):453–461. doi: 10.1016/j.ajhg.2014.01.006

Table 3.

CA-VA Wild-Type and p.Ser233Pro Mutant Enzyme Activities in COS-7 Cell Lysates, before and after Incubation at Human Body Temperature

Enzyme EU/mg Cell Protein before Heating EU/mg Cell Protein after Heating (30 min at 37°C)
WT 1.02 (100%) 1.25 (100%)
MT 0.15 (14.7%) 0.07 (5.4%)

The enzyme activities were determined in duplicate and calculated as enzyme unit (EU) per milligram (mg) of enzyme protein. Incubation was at 37°C for 30 min. Abbreviations are as follows: WT, wild-type; MT, mutant.