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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Plant J. 2014 Feb 11;77(5):667–675. doi: 10.1111/tpj.12422

Figure 2.

Figure 2

Kinetic characterization of CD1/SlCUS1. (a) The pH optimum of CD1/SlCUS1 assayed at 37°C for 2 h in buffers of respective pH values. Buffers used were sodium acetate, closed circles; sodium phosphate, open circles; Tris-HCl, closed triangles. (b) The temperature optimum of CD1/SlCUS1 assayed for 2 h at the respective temperatures in pH 5.0 buffer. (c) The effect of 2-MHG concentration on initial reaction rate. Data were fit to the Michaelis-Menten model as shown. Error bars represent standard deviation for triplicate reactions.