Skip to main content
. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Ann Allergy Asthma Immunol. 2014 Jan 7;112(3):211–216.e1. doi: 10.1016/j.anai.2013.12.006

Figure 3.

Figure 3

Addition of LTB4 to stimulated CD8+ T cells results in increased intracellular Ca2+ concentrations. Isolated CD8+ T cells stimulated with anti-CD3/anti-CD28 for 8 days were incubated with LTB4 (100 or 1,000 nM) and intracellular Ca2+ concentrations were monitored comparing the median fluoresense ratio of indo-1 to indo-2 as described in Methods.