Figure 3.
Addition of LTB4 to stimulated CD8+ T cells results in increased intracellular Ca2+ concentrations. Isolated CD8+ T cells stimulated with anti-CD3/anti-CD28 for 8 days were incubated with LTB4 (100 or 1,000 nM) and intracellular Ca2+ concentrations were monitored comparing the median fluoresense ratio of indo-1 to indo-2 as described in Methods.