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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biomaterials. 2013 Sep 14;34(37):9295–9306. doi: 10.1016/j.biomaterials.2013.08.054

Figure 4.

Figure 4

Examination of scaffold structure, cell morphology and distribution by SEM (A–F) and H&E staining (G–L). (A, B, D, E) hASCs attached to both types of scaffold 1 day after seeding. Cells in the tECM-supplemented scaffolds exhibit aligned cell bodies and extensions along the longitudinal axis of the scaffold (D, white arrows), whereas cells in the pure collagen scaffold are more randomly oriented (A, white arrows). (B, E) Higher magnification images show more extensions from the cell body attached to the collagen fibrils in tECM-supplemented scaffolds (E, white arrows) compared to pure collagen scaffolds (B, white arrows). (C, F) After 7 days of culture, cell density in each group is dramatically higher than that of day 1, and most cells assume an elongated spindle shape along the longitudinal axis of the scaffold. (G, J) H&E staining demonstrates uniform cell distribution along the depth of the constructs 1 day after gel setting. (H, I, K, L) At day 7, cells in each group are longitudinally aligned. (I, L) Interestingly, intense hematoxylin nuclear staining is evident at the peripheral region of tECM-supplemented scaffolds (L, black arrows).