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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Chem Sci. 2013 Oct 31;5(2):507–514. doi: 10.1039/C3SC52019F

Fig. 4.

Fig. 4

2 binds 1 equivalent of heme B per 4-helix bundle. (A) UV/visible spectra from the titration of 0.5 μM hemin aliquots (0.5 mM hemin in DMSO) into 5 μM 2 (1 cm pathlength cuvette, 25 °C, 20 mM phosphate, 100 mM KCl, pH 7.5). (B) Heme binding isotherm indicating a stoichiometry of 1 heme B per molecule of 2 (dashed line).