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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Kidney Int. 2013 Sep 11;85(4):807–823. doi: 10.1038/ki.2013.345

Figure 2. Conditional deletion of renal endothelial S1P1R in S1P1Rf/f PdgfbiCreER mice.

Figure 2

Figure 2

S1P1–5R mRNA levels measured with RTPCR in freshly isolated renal endothelial cells (with Dynabeads coupled to CD144 antibody) and renal proximal tubules (with Percoll density gradient separation) from conditional S1P1R null mice (S1P1Rf/f PdgfbiCreER) and wild type mice (S1P1Rf/f). Representative gel image of PCR products (A) and densitometric quantification of relative band intensities normalized to GAPDH (B, N=4). Endothelial cells from conditional endothelial S1P1R null mice show significantly (∼90%) reduced S1P1R mRNA expression compared to wild type mice without any changes in other S1PR subtype (S1P2–5R) mRNA expression (N=4). Furthermore, proximal tubule S1P1R mRNA levels were similar between conditional endothelial S1P1R null mice and wild type mice. *P<0.05 vs. S1P1Rf/f mice.