Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: Kidney Int. 2013 Sep 11;85(4):807–823. doi: 10.1038/ki.2013.345

Representative gel images (A) of RTPCR and densitometric quantification of relative band intensities normalized to GAPDH (B) of pro-inflammatory markers TNF-α, ICAM-1, MCP-1 and MIP-2 in kidneys from tamoxifen-treated (5 mg/kg i.p. daily for 3 days) endothelial S1P₁R null (S1P₁R^f/f Pdgfb^iCreER) or wild type (S1P₁R^f/f) mice. Mice were subjected to sham-operation or to 20 min of renal ischemia followed by 24 hr of reperfusion (N=4–5 per group). Conditional deletion of endothelial S1P₁R significantly increased the expression of TNF-α, MCP-1 and ICAM-1 mRNAs examined compared to the wild type mice subjected to renal IR. *P<0.05 vs. Sham-operated mice. #P<0.05 vs. wild type mice subjected to renal IR. Error bars represent 1 SEM.

Representative gel images (A) of RTPCR and densitometric quantification of relative band intensities normalized to GAPDH (B) of pro-inflammatory markers TNF-α, ICAM-1, MCP-1 and MIP-2 in kidneys from tamoxifen-treated (5 mg/kg i.p. daily for 3 days) endothelial S1P₁R null (S1P₁R^f/f Pdgfb^iCreER) or wild type (S1P₁R^f/f) mice. Mice were subjected to sham-operation or to 20 min of renal ischemia followed by 24 hr of reperfusion (N=4–5 per group). Conditional deletion of endothelial S1P₁R significantly increased the expression of TNF-α, MCP-1 and ICAM-1 mRNAs examined compared to the wild type mice subjected to renal IR. *P<0.05 vs. Sham-operated mice. #P<0.05 vs. wild type mice subjected to renal IR. Error bars represent 1 SEM.