Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: Kidney Int. 2013 Sep 11;85(4):807–823. doi: 10.1038/ki.2013.345

Representative gel images of RTPCR (A) and densitometric quantification of relative band intensities normalized to GAPDH (B) of HSP27, HO-1 (HSP32) and HSP70 mRNA expression in freshly isolated endothelial and proximal tubule cells from conditional endothelial S1P₁R null mice or from wild type (S1P₁R^f/f) mice. Renal endothelial cells from endothelial S1P₁R null mice express significantly reduced HSP27 mRNA compared wild type mice without any changes in HO-1 or HSP70 expression. Furthermore, we show that HSP27, HSP32 and HSP70 mRNA levels in freshly isolated proximal tubule cells were similar between endothelial S1P₁R null mice and wild type mice. C and D. Representative gel images of RTPCR (top) and densitometric quantification of relative band intensities normalized to GAPDH (bottom) of HSP27 mRNA expression in freshly isolated endothelial cells from C57BL/6 mice treated for 3 days (C) or for 1 hr (D) with vehicle or with W146 (a selective S1P₁R antagonist, 0.1 mg/kg). S1P₁R antagonist treatment significant reduced endothelial HSP27 expression only after treatment for 3 days with W146 (C). W146 treatment for 1 hr had no effect on endothelial HSP27 expression (D). *P<0.05 vs. wild type (S1P₁R^f/f) mice or vehicle-treated C57BL/6 mice.

Representative gel images of RTPCR (A) and densitometric quantification of relative band intensities normalized to GAPDH (B) of HSP27, HO-1 (HSP32) and HSP70 mRNA expression in freshly isolated endothelial and proximal tubule cells from conditional endothelial S1P₁R null mice or from wild type (S1P₁R^f/f) mice. Renal endothelial cells from endothelial S1P₁R null mice express significantly reduced HSP27 mRNA compared wild type mice without any changes in HO-1 or HSP70 expression. Furthermore, we show that HSP27, HSP32 and HSP70 mRNA levels in freshly isolated proximal tubule cells were similar between endothelial S1P₁R null mice and wild type mice. C and D. Representative gel images of RTPCR (top) and densitometric quantification of relative band intensities normalized to GAPDH (bottom) of HSP27 mRNA expression in freshly isolated endothelial cells from C57BL/6 mice treated for 3 days (C) or for 1 hr (D) with vehicle or with W146 (a selective S1P₁R antagonist, 0.1 mg/kg). S1P₁R antagonist treatment significant reduced endothelial HSP27 expression only after treatment for 3 days with W146 (C). W146 treatment for 1 hr had no effect on endothelial HSP27 expression (D). *P<0.05 vs. wild type (S1P₁R^f/f) mice or vehicle-treated C57BL/6 mice.