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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Adv Biosci Biotechnol. 2013 Jul 15;4(8C):30–37. doi: 10.4236/abb.2013.48A3004

Figure 2.

Figure 2

MDP-mediated sensitization of RAW 264.7 macrophages to subsequent stimulation with C5a is dependent on NOD2 expression. RAW 264.7 macrophages were treated with or without MDP (1 µg/mL) for (A) 90 min or (B) 16 h. Cells were then challenged with C5a (10 ng/mL) for 90 min and expression of MCP-1 and MIP-2 mRNA measured by qRT-PCR. (C) RAW 264.7 macrophages were nucleofected with 200 nM of Nod2 siRNA or scrambled siRNA. The efficiency of siRNA was assessed by mesauring NOD2 mRNA. After 32 h, cells were then treated with or without MDP (1 µg/mL) for an additional 16 h. Cells were then challenged with C5a (10 ng/mL) for 90 min and expression of MCP-1 and MIP-2 mRNA measured by qRT-PCR. Values represent means ± SEM, n = 3–5. (A/B) Values with different superscripts are significantly different from each other, p < 0.05. (C) *p < 0.05 compared to cells transfected with scrambled siRNA.