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QDO, SD medium without Trp, Leu, His and Ade; QDO/X, QDO medium with X-α-Gal. 1.pGBKT7-53 & pGADT7-T (positive control); 2.pGBKT7-Lam & pGADT7-T (negative control); 3.pGBKT7-FYFL & pGADT7-RIN; 4.pGBKT7-FYFL & pGADT7- MADS1; 5.pGBKT7-FYFL & pGADT7-JOINTLESS; 6.Blank. Empty bait vector, empty prey vector and autoactivation assay were growth of none yeast.
