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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: J Immunol. 2014 Feb 12;192(6):2892–2903. doi: 10.4049/jimmunol.1302847

Table I. Foxp3 overexpression increases endogenous MHC Class I expression in Jurkat cells.

Jurkat cells were transfected with Foxp3 cDNA (2.0 ug/ 106 cells); after 24 hrs, cells were surface stained either with anti-class I antibody and secondary antibody or secondary alone, as a control. Foxp3+ transfectants were distinguished from non-transfectants by intracellular staining with anti-Foxp3. Both cell surface and intracellular staining levels were assayed by flow cytometry. Transfection efficiency was 10%.

Class I* Control*
(secondary antibody only)
Foxp3+ 318 11.3
Foxp3− 70.5 8.25
*

MFI