Adenoviral transgene expression in the MB49 orthotopic model in the absence and presence of the NPGDE-1,4 Bis polymer. Luciferase activity in bladder homogenates from mice 24 hours after intravesical delivery of PBS, AdLuc or AdLuc plus NPGDE-1,4 Bis polymer. AdLuc and NPGDE-1,4 Bis were given at was 1 × 109 PFU and 400 μg, respectively. Circles represent data points and “x” indicates the estimated mean. P-values were less than 0.001 in comparisons of PBS vs. AdLuc, PBS vs. AdLuc plus NPGDE-1,4 Bis polymer and AdLuc vs. AdLuc plus NPGDE-1,4 Bis polymer.