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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Cancer Res. 2013 Dec 16;74(4):1045–1055. doi: 10.1158/0008-5472.CAN-13-2908

Figure 5.

Figure 5

Ex vivo expression of PD-1 and Tim-3 by NY-ESO-1 157-165-specific CD8+ T cells following immunizations in arms 1 and 2. (A-B) Flow cytometry dot plots from one selected melanoma patient in arm 2 (MP6) (A), and summary data for all patients vaccinated in arm 1 (n = 5) and arm 2 (n = 7) (B), showing ex vivo percentages of PD-1+ and Tim-3+ cells within total NY-ESO-1 tet+ CD8+ T cells, at different time points throughout the course of vaccination. Vertical lines, time points of vaccinations. (C) Correlation between the fold changes in NY-ESO-1 tet+ CD8+ T cell frequencies and the differences in ex vivo PD-1 and Tim-3 expression (differences in percentage and MFI of PD-1 and Tim-3 expression by NY-ESO-1 tet+ CD8+ T cells) throughout the course of vaccination. P < 0.05 was considered significant. Data shown are from two independent experiments.