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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2006 Nov 9;292(3):G785–G795. doi: 10.1152/ajpgi.00293.2006

Table 3.

Kinetic parameters of human recombinant NTPDase8

Substrate Km, μM Vmax, nmol Pi·min−1 · mg−1
ATP 81±7 790±35
ADP 137±13 163±9
UTP 480±32 1100±50
UDP 241±17 110±4

Human NTPDase8 exhibited Michaelis-Menten kinetics for the hydrolysis of ATP, ADP, UTP, and UDP. Apparent Km and Vmax values were estimated from Eadie-Hofstee plots created with GraphPad Prism software (GraphPad Software, San Diego, CA). Results are expressed as means ± SE of 3 separate experiments, each performed in triplicate.